ABSTRACT
Objective To study the expressions of Hepcidin,bone morphogenetic protein 6 (BMP6) and hemojuvelin (HJV) in gastric cancer,and to explore their relationships with clinical pathological characteristics.Methods Hepcidin,BMP6 and HJV were detected by immunohistochemistry in 62 cases of gastric cancer patients,and the relationships among them and clinical pathological features were analyzed.Results Compared with normal gastric mucosa tissues,Hepcidin was highly expressed in gastric cancer tissues (13.3％ vs.56.5％,x2 =8.99,P ＜ 0.01),while the differences of the expression of BMP6 and HJV in the two groups were not statistically significant (60.0％ vs.40.3 ％,x2 =1.89,P ＞ 0.05;93.3％ vs.83.9％,x2 =0.88,P ＞ 0.05).The expression of Hepcidin was related to T stage (x2 =6.98,P =0.02),but it was not related to age,sex,lymph node metastasis,distant metastasis and anemia.The expressions of BMP6 and HJV were not related to T stage,age,sex,lymph node metastasis,distant metastasis and anemia.Hepcidin was not related to the expressions of BMP6 and HJV (r=0.13,P＞0.05;r=0.15,P＞0.05).Conclusion Hepcidin is highly expressed in gastric cancer tissues,which is related to the T staging of gastric cancer,and can be used as an objective indicator of the biological behavior of gastric cancer.There were no differences in the expression of BMP6 and HJV between normal gastric mucosa and gastric cancer tissues.
ABSTRACT
La hepcidina es una hormona peptídica, producida en el hígado y considerada unregulador del metabolismo del hierro. Su regulación está dirigida a la absorciónintestinal del hierro y a la función de la ferroportina dentro de la célula, a fin demantener un balance entre el consumo y las reservas de hierro. Esta hormona, a suvez, está regulada por procesos inflamatorios, estados de anemia ferropénica, actividadhematopoyética y algunas vías de señalización como SMAD, STAT y JAK. En estasvías interviene una proteína de membrana llamada hemojuvelina, la cual actúa comoun correceptor de la proteína morfogenética ósea, cuya función es inducir el gende transcripción de la hepcidina mediante la vía SMAD1/5/8-SMAD4. Debido a laasociación de la hemojuvelina con la expresión de la hepcidina y la relación de estaúltima con procesos inflamatorios y regulación del metabolismo del hierro, se hanpropuesto algunas técnicas para la determinación de ambas proteínas como Elisa,Dotblot, inmunoensayos y SELDI-TOF MS...
The hepcidin is a peptide hormone produced by theliver with anti-bacterial activity, is involved in regulationiron metabolism, making it possible to useit as another parameter to assess some entities relatedto disorders associated with iron metabolism.Hepcidin regulates intestinal absorption of ironlevel and the role of ferroportin in the cell, preventingeither iron overload or iron consumptionreserve, this is turn regulating for inflammatorystimuli, iron store, erythropoietic activity andsignaling patway SMAD,STAT and JAK; in thissignaling patway involved a membrane proteincalled hemojuvelin. The hemojuvelin acts as abone morphogenetic protein co-receptor, theyinduce hepcidin gene transcription through theSMAD1/5/8-SMAD4. Because of the associationsof hemojuveline with hepcidin expression andthe relationship of the latter with inflammatoryprocesses and regulations of iron metabolismhave been proposed some techniques for the determinationof both proteins as Elisa, Dotblot,Immunoassays and SELDI-TOF MS...
Subject(s)
Intestinal Absorption , Iron/adverse effects , Iron/physiologyABSTRACT
Juvenile hemochromatosis is an autosomal recessive disease characterized by progressive tissue iron overload which leads to irreversible organ damage and even death.This disease is mainly caused by mutations in two genes:hemojuvelin gene and hepcidin gene.Different mutations have different phenotype.The two genes may act as modifying genes in HFE hemochromatosis.Hepcidin secreted by liver plays a central role in the regulation of iron homeostasis.HJV can act as a bone morphogenetic protein(BMP)co-receptor which is required for HJV to regulate hepcidin expression and iron homeostasis.Recent researches suggest that the bone morphogenetic protein(BMP)signaling pathway mediated by HJV is a significant mechanism for HJV to regulate hepcidin expression and iron homeostasis.HJV mutant impaires BMP signaling which results in hepcidin expression decrease and abnormal iron metabolism.